1. Western blotting is the technique for the detection of
a) specific DNA in a sample
b) specific RNA in a sample
c) specific protein in a sample
d) specific glycolipid in a sample
2. Which of the following technique is used in DNA finger printing?
a) Western blotting
b) Southern blotting
c) Northern blotting
d) Eastern blotting
3. Arrange the following in correct order
1. Southern Blotting ----A)Alwine
2. Western blotting ----B)E.M.Southern
3. Northern Blotting----C) A. Jeffrey
4. DNA fingerprinting---- D) Towbin
a) 1-A, 2-C, 3-D, 4-B
b) 1-B, 2-D, 3-A, 4-C
c) 1-B, 2-A, 3-D, 4-C
d) 1-B, 2-C, 3-A, 4-C
4. Labelled Antibodies are used to detect
a) detect the presence of a particular DNA molecule in Southern blotting
b) detect the presence of a particular RNA molecule in Southern blotting
c) detect the presence of a particular protein molecule in Southern blotting
d) detect the presence of a particular protein molecule in Western blotting
5. Which of the following technique is suitable for identifying mRNA molecule in a sample
a) Western blotting
b) Southern blotting
c) Northern blotting
d) Eastern blotting
6. Which of the following technique doesn’t involve electrophoresis for the separation of biomolecules
a) Dot blotting
b) Southern blotting
c) Northern blotting
d) Western blotting
7. Probe is a
a) protein for detecting a specific DNA molecule
b) short piece of labelled DNA which are complementary to the nucleic acid strand to be detected
c) short piece of labelled DNA or RNA which are complementary to the nucleic acid strand to be detected
d) none of these
8. Which of the following technique is most suitable for detecting the presence of a gene product
a) Dot blotting
b) Southern blotting
c) plaque blotting
d) Western blotting
9. Aminobenzyloxymethyl filter paper is commonly used for transfer in
a) Western blotting
b) Southern blotting
c) Northern blotting
d) Dot blotting
10. Arrange the following in correct order
1. Southern Blotting ----A)RNA-DNA hybrid
2. Western blotting -----B)DNA-DNA hybrid
3. Northern Blotting-----C)Southern blotting
4. DNA fingerprinting---D)antigen antibody reaction
a) 1-A, 2-C, 3-D, 4-B
b) 1-B, 2-D, 3-A, 4-C
c) 1-B, 2-A, 3-D, 4-C
d) 1-B, 2-C, 3-A, 4-C
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Answers
1. c) specific protein in a sample
2. b) Southern blotting
3. b) 1-B, 2-D, 3-A, 4-C
4. d) detect the presence of a particular protein molecule in Western blotting
5. c) Northern blotting
6. a) Dot blotting
7. c) short piece of labelled DNA or RNA which are complementary to the nucleic acid strand to be detected
8. d) Western blotting
9. c) Northern blotting
10. b) 1-B, 2-D, 3-A, 4-C
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